This is a simplified quick start procedure for the calculation of results using Model 394 Trace Analysis System.

1. Preparation. Obtain blank, sample, and standards for analysis using your standard method of preparation.

2. Analysis. If required, analyze duplicate replicates of the blank and the sample by your method. If you are analyzing duplicate replicates, you must run a scan for each replicate and store the results of each curve in a separate file. If the method of standard addition is employed, then spike each replicate sample with a known amount before the second scan for the standard response. Alternatively, prepare a simple standard curve and store the results of each curve in a separate file.

3. Replicates. (For duplicate replicate analysis only) Recall one of the two duplicate curves into a screen. Choose Tools, File Math, Add. Browse the data files and choose the second file of the sample curve. Choose Tools, Data Math, / Constant. Enter the number, "2", and calculate the average of the two curves. Store the calculated curve into a new file. Perform this procedure for each pair of replicates.

4. Calibration. Clear all peaks from the screens. Recall one of the standard addition or standard curve files, choose Results, Peak Search. Study the plot carefully and note the peak number corresponding to the analyte peak(s) of interest. You may be able to identify as many as nine peaks for standards calibration and analysis. Choose View, Data Setup, Sample/Cell, and see the list of components 1?9 on the left side. Enter the concentration of the standard next to the previously noted peak(s). (If the concentration units are not correct, then go to Options, Experiment, Concentration and select proper units. Click on OK and exit) Click on OK. Notice that the entered concentration now appears in the right column under the appropriate peak. If you care to identify each peak with an alphanumeric code on the graphical display, then choose Results, Analyze, and enter the code in the upper row of boxes corresponding to the peak numbers 1 to 3. Use the scroll bar near the bottom of the screen to access to the other six peaks, if any. Click on OK and exit the Results/Analysis Screen and save the file. All entries will be saved and presented each time the curve file is recalled. Repeat for each standard curve to be used in the calibration curve.

5. Results setup. Next, choose Results, Analyze to get to the Standards/Analysis Table. Observe that the file you had last recalled is shown in the sample file box. Check the Peak Locate, and/or Blank Subtract Boxes, as necessary. Choose Standard Curve or Standard Addition Method box. Choose Blank Subtract and Peak Location, if desired. If you had chosen peak location option, then the top table will list peaks identified by potential (mV) and peak number. Also, you will see the recalled data file listed in the Sample box under the filename column along with the measured peak height listed in the Size columns below each numbered peak. If blank subtract is chosen, then sample and standard peaks will have the blank file subtracted from each for the purposes of the calculation of the concentration in this table. (Alternatively, you may choose Tools, File math, Subtract to permanently correct the sample and standard curves for a blank. We do not recommend this procedure unless one is certain of the consistency of blank response from sample to sample.)

6. Curve file entry. Click on the Filename box on the left column for the blank, choose Browse and the blank curve file. The file name should appear in the blank box. Repeat this procedure for each standard (Std1, Std2, .....Std9) by clicking in the appropriate box and Browsing in the file. (Make sure that you click in the appropriate target box before browsing in the file, otherwise you will read all the files into the same box.). When all curves are entered in the Results/Analysis Table, choose Find, Calc. The calculated peak heights should appear in each row along side the concentrations of each standard curve that was entered earlier .

7. Calculation of sample concentrations. Next, click in the sample box and Browse in a sample curve. Choose Find, Calc. Calculated results for all previously identified peaks in the sample file will appear in their respective positions in sample box row. Choose Calib Plot, enter desired peak number, and click on OK for the calibration plot.

8. Storage of calibration table. If one wishes to store the standard curve calibration table for future calculations, choose File, Save As, and the appropriate file name. A suffix of *.ANS will be added to the file and stored.

9. Plotting of results, graph, etc. Click on File, Page Setup. Check boxes for Data Setup Parameters, Analysis Results, Graph, Graph/Analysis Results as desired. When File, Print is chosen, all of the items in the checked boxes will be printed.